MS-Ready Antibody Screening in 10 Minutes

Monoclonal antibody screening often slows down at the sample-preparation step. Before LC-MS or top-down MS can start, labs typically need several manual operations after protein A capture: elution at low pH, neutralization, desalting, buffer exchange and concentration adjustment. Each additional step costs time dilutes the sample and can introduce variability.

Digital membrane chromatography changes that workflow. Instead of using harsh pH shifts or high salt to release the antibody from a protein A membrane, the system uses voltage as the elution trigger. This allows capture and release under gentle conditions and in MS-compatible buffers. The result is a highly streamlined preparation workflow that can deliver concentrated, mass spectrometry-ready antibody samples in about 10 minutes.

For analytical labs, this means less hands-on work and fewer transfer losses. SEC, desalting and rebuffering steps can be reduced or eliminated, depending on the application. Because the membrane format is fast by design, the method combines the binding selectivity of protein A with the speed of membrane chromatography and the controllability of an electronic system.

The approach is especially relevant for high-throughput antibody screening, clone selection and bioprocess monitoring. Faster preparation enables earlier access to molecular information and shortens the path from sample to decision. At the same time, the gentle elution conditions help preserve sample quality for more demanding downstream analyses.

With digital membrane chromatography, protein A purification is no longer just a capture step. It becomes a direct bridge to mass spectrometry: fast, gentle, concentrated and designed for modern analytical workflows.